Mitosis: KLP61F Goes Wee!
نویسندگان
چکیده
Kinesin-5s help assemble the bipolar spindle by crosslinking and sliding apart antiparallel microtubules. A recent study has uncovered a novel pathway for the phospho-regulation of these motors.
منابع مشابه
BimC motor protein KLP61F cycles between mitotic spindles and fusomes in Drosophila germ cells
KLP61F in Drosophila is a member of the BimC family of kinesins and, as for other family members [1], is required for spindle assembly [2] [3]. KLP61F is a bipolar homotetramer that cross-links spindle microtubules [4]. It is not known, however, whether the function of KLP61F is dedicated to mitosis or whether KLP61F interacts exclusively with microtubules. Previous work suggested that KLP61F f...
متن کاملKinesin-5-dependent poleward flux and spindle length control in Drosophila embryo mitosis.
We used antibody microinjection and genetic manipulations to dissect the various roles of the homotetrameric kinesin-5, KLP61F, in astral, centrosome-controlled Drosophila embryo spindles and to test the hypothesis that it slides apart interpolar (ip) microtubules (MT), thereby controlling poleward flux and spindle length. In wild-type and Ncd null mutant embryos, anti-KLP61F dissociated the mo...
متن کاملDynamic partitioning of mitotic kinesin-5 cross-linkers between microtubule-bound and freely diffusing states
The dynamic behavior of homotetrameric kinesin-5 during mitosis is poorly understood. Kinesin-5 may function only by binding, cross-linking, and sliding adjacent spindle microtubules (MTs), or, alternatively, it may bind to a stable "spindle matrix" to generate mitotic movements. We created transgenic Drosophila melanogaster expressing fluorescent kinesin-5, KLP61F-GFP, in a klp61f mutant backg...
متن کاملThe kinesin-like protein KLP61F is essential for mitosis in Drosophila
We report here that disruption of a recently discovered kinesin-like protein in Drosophila melanogaster, KLP61F, results in a mitotic mutation lethal to the organism. We show that in the absence of KLP61F function, spindle poles fail to separate, resulting in the formation of monopolar mitotic spindles. The resulting phenotype of metaphase arrest with polyploid cells is reminiscent of that seen...
متن کاملAn MBoC Favorite: Functional coordination of three mitotic motors in Drosophila embryos
It is well established that multiple microtubule-based motors contribute to the formation and function of the mitotic spindle, but how the activities of these motors interrelate remains unclear. Here we visualize spindle formation in living Drosophila embryos to show that spindle pole movements are directed by a temporally coordinated balance of forces generated by three mitotic motors, cytopla...
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عنوان ژورنال:
- Current Biology
دوره 19 شماره
صفحات -
تاریخ انتشار 2009